3. Results
The overall AAFEs was 1.25 (ranged: 1.08–1.59) (Eron et al., 2004; Hsu et al., 1997; Mzayek et al., 2007; Na-Bangchang et al., 1994). The AAFEs for ritonavir, lopinavir (LPV/r), and chloroquine were 1.30 (Hsu et al., 1997), 1.17 (Eron et al., 2004) and 1.27 (Mzayek et al., 2007; Na-Bangchang et al., 1994), respectively. All AAFEs values were within the accepted ranges (Table S2). Sensitivity coefficients for Ka, fu, Rbp, fm, CYP3A4, and fm, CYP2D6 were +0.13, -0.90, +0.06, -0.50, and +1.34, respectively. All sensitivity coefficients except for fm,CYP2D6 were less than one, indicating no significant effects of the model parameters on the AUC values. Although fm,CYP2D6 was greater than one, this value was assumed based on the intrinsic clearance of chloroquine from the vitro studies.7 All parameters were within the accepted ranges, indicating model reliability.