Materials
AT-403
(2-(1-(1-((1s,4s)-4-isopropylcyclohexyl)piperidin-4-yl)-2-oxoindolin-3-yl)Nmethylacetamide)
was synthesized by Dr NT Zaveri at Astraea Therapeutics (Mountain View,
CA, USA) (Arcuri et al. , 2018).
CCG-203920
(4-(2-(methoxyethyl-)-2-ethyl-1,2,4-thiadiazolidine-3,5-dione) was
synthesized by the Medicinal Chemistry Core at Michigan State University
(East Lansing, MI, USA). L-Dopa and benserazide were purchased from
Sigma-Aldrich (Milan, Italy). 6-OHDA hydrobromide and N/OFQ were
purchased from Tocris Bioscience (Bristol, UK). U1079 antibody was a
generous gift of Dr S Mumby (University of Texas Southwestern, Dallas,
TX, USA). AT-403 was dissolved in 2% CH3COOH 1M and 4% DMSO water,
L-DOPA, benserazide and CCG-203920 were dissolved in saline, 6-OHDA was
dissolved in saline with 0.02% ascorbic acid. N/OFQ and SKF-38393 were
dissolved in water. LANCE Ultra cAMP assay was purchased from Perkin
Elmer (Waltham, MA). All plasmids were purchased from cDNA Resource
center (Bloomsburg, PA, USA).