Cytokine detection
The serum was obtained by centrifugation at 1000 × g for 10 min and the
content of IL-35 was detected by an AimPlex bead-based immunoassay
(Quanto Bio, Beijing, China) according to the manufacturer’s
instructions. All samples were analyzed by a FACS Calibur instrument and
data were collected by Cell Quest Pro software (BD Biosciences, San
Diego, CA, USA). Data were analyzed by FCAP Array V3.0 software (BD
Biosciences).