Chromatin immunoprecipitation
Chromatin immunoprecipitation assays were conducted according to the previously described protocol (Lin et al. , 2012). Briefly, MIN6 cells were lysed and sonicated to generate DNA fragments of 300–1000 nucleotides. Protein-linked DNA was immunoprecipitated with PPARγ antibody or control rabbit IgG, and the immunoprecipitated DNA sequence was then examined by quantitative PCR. The primers used in chromatin immunoprecipitation were listed in Table S2. The amount of the target DNA immunoprecipitated was normalized to a control input.