Figure 7. PPARγ regulated the expression of Heparanase
(Hpse) gene. (A) The predicted sequence of PPARγ binding sites in the
promoter region of Hpse (2000 bases forward TSS, -2000/0)
according to JASPAR database. PPRE1, -1567/-1552; PPRE2, -442/-427;
PPRE3, -384/-369. (B) The predicted sequence of PPRE according to JASPAR
database. (C) Luciferase activity of pGL3 with different lengths of the
promoter region of Hpse genes (-2000/0), pGL3 (-1,000/0), pGL3
(-300/0), and pGL3 basic reporter plasmids co-transfected with PPARγ in
293T, and rosiglitazone (ROSI) was used to activate PPARγ.
**p< 0.01, ***p< 0.001, ns=not significant as
indicated, n=6. (D) Interaction of the PPARγ and the promoter region ofHpse was detected by chromatin immunoprecipitation in 293T;
rosiglitazone was used to activate PPARγ. (n=6). *p< 0.05 in
PPARγ antibody (Ab) treated group compared to input control,
#p<0.05 in ROSI-stimulated group compared to DMSO-treated
control.