OGT2115 did not protect pancreatic beta cells from STZ -induced
dysfunction in vitro
We used the mouse insulinoma MIN6 cell line and primary isolated murine
islets to test whether OGT2115 had a protective effect in cultured beta
cells in vitro . The MTT assay showed that a high dosage of
OGT2115 (more than 10 μM) could significantly disrupt the viability of
MIN6 cells (IC50=10.31 μM, Fig. 6A). Concentrations of up to 1 μM
OGT2115 were used in further in vitro experiments. STZ reduced the
viability of MIN6 cells after a 24 h incubation. However, co-incubation
with OGT2115 did not prevent the damage by STZ (Fig. 6B) on MIN6 cells.
Likewise, OGT2115 did not protect against STZ-induced damage in the GSIS
of cultured islets (Fig. 6C). The STZ- induced reduction in mRNA level
of genes involved in insulin expression and secretion, includinginsulin , Pdx-1 and Mafa , were not improved by
OGT2115 treatment of cultured islets (Fig. 6D).