Chromatin immunoprecipitation
Chromatin immunoprecipitation assays were conducted according to the
previously described protocol (Lin et al. , 2012). Briefly,
MIN6 cells were lysed and sonicated to generate DNA fragments of
300–1000 nucleotides. Protein-linked DNA was immunoprecipitated with
PPARγ antibody or control rabbit IgG, and the
immunoprecipitated
DNA sequence was then examined by quantitative PCR. The primers used in
chromatin immunoprecipitation were listed in Table S2. The amount of the
target DNA immunoprecipitated was normalized to a control input.