High-performance liquid chromatography (HPLC) analysis of tanshinones and GA3 contents
The contents of tanshinones and GA3 were determined by HPLC, according to the general method in our laboratory that was described previously (Wang et al. , 2008; Liu et al. , 2016). 0.04 g dried hairy roots power were extracted by 8 mL of 70% methanol overnight. After centrifugation, the supernatant was filtered through a 0.2-μm filter for HPLC analysis.