Dual-luciferase assay
The 649-bp promoter of SmKSL1 was cloned and inserted intopGREEN . The vector pCAMBIA1304-SmGRAS5 was transferred
into Agrobacterium strain GV3101. pCAMBIA1304 empty vector
was used as a negative control and The 35S promoter-drivenRenilla luciferase as an internal control. The two GV3101 strains
were co-infiltrated into tobacco leaves. Infiltrated leaves were
incubated in darkness for 8h and then in light for 40h. Three biological
replicates of each sample were assayed using the Dual-Luciferase
Reporter Assay System (Promega, USA).