Construction of RNA-seq libraries and transcriptome data
analysis
21-day-old transgenic hairy roots (G5O14) and control roots (ATCC) were
collected from three biological replicates and used for total RNA
extraction. 3 μg RNA of each sample was used to construct Illumina
sequencing libraries using the Illumina PE150 platform (Novogene,
China.). Three biological replicates were used for each sample.
The reference genome and gene model annotation files were downloaded
from genome websites (Xu et al. , 2016). Genes with an adjustedP -value<0.05 and |fold-change|≥2 were
considered significantly differentially expressed. GO Slim enrichment
was evaluated using the GOseq R package (Young et al. , 2010).
Significantly enriched KEGG pathways were identified with KOBAS 2.0 (Maoet al. , 2005).