Dual-luciferase assay
Protoplasts were isolated, and the dual-luciferase assay was performed
as previously described (Han et al., 2017). Transformed protoplasts were
incubated at room temperature (~22℃) for 20-22 h and
luciferase activities were measured using the dual-luciferase assay
system (Dual-Luciferase® Reporter Assay, Promega,
United States) according to the manufacturer’s instructions. Firefly
luciferase activity was normalized to Renilla luciferase
activity. Three biological replicates were performed for all
experiments.