Fig. 7 COL13 promotes the interaction between COL3 and COP1.
(a) Yeast three-hybrid analysis of COP1-COL3 interaction in the presence of COL13. Normalized Miller units were calculated as a ratio of α-galactosidase activity in yeast. Additionally, normalized Miller units are reported separately for yeast grown on media with or without 1 mM methionine (Met), corresponding to induction (-Met) or repression (+Met) of Met25 promoter-driven COL13 expression, respectively. Means and standard errors of the means for three biological repetitions are shown. Lower-case letters indicate significant differences in α-galactosidase. (b) A model representing the HY5-COL3-COL13 regulatory chain and COP1-dependent COL3-COL13 feedback pathway in the regulation of hypocotyl elongation.