2.6. Phylogenetic analysis.
Phylogenetic analysis was performed using MEGA X (Kumar et al., 2018) that relies on the Maximum Likelihood method and JTT matrix-based model. The trees with the highest log likelihood (A: -11895.94, B: -7003.22, C: -18522.86) are shown. The tree was obtained applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value (Jones et al., 1992). These analysis involved (A: 8, B: 8, C: 20) amino acid sequences respectively. There were a total (A: 1124, B: 947, C: 752) positions respectively in the final dataset. Phylogenetic tree analysis for SPS and SS orthologues was performed usingArabidopsis protein sequences, selecting only the closest ones between both species without analyzing the furthest clusters. Regarding invertases, the phylogenetic tree performed for CWINV orthologues was using protein sequences with 6-FEH activity from Arabidopsis : AtCWINV1 (At3G13790), AtCWINV3 (At1G55120), AtCWINV5 (At3G13784) andBeta vulgaris : Bv6-FEH (AJ508534); 1-FEH activity fromCichorium intybus Ci1-FEH (AJ242538), Ci1-FEH2a (AJ295033); and 6-1-FEH activity from Arabidopsis AtCWINV6 (At5G11920) andTriticum aestivum Ta6-1-FEH (AB089269). For VINV orthologues was using the closest protein sequences from Arabidopsis : AtVI2 (AT1G12240), AtVI1 (AT1G62660); protein sequences with 1-SST activity from Allium cepa : Ac1-SST (AJ006066), Cichorium intybus : Ci1-SST (U81520), Helianthus tuberosus Ht1-SST (AJ009757); and protein sequences with 6-GFT activity from Allium cepa : Ac6-GFT (ACY07838) and Asparagus officinalis Ao6-GFT (AB084283). Accession numbers used for all analyses are according to the following databases nomenclature respectively (gene_V4, gene_V2, NCBI_LOC, NCBI_PROT) (Supplemental Information S1).