Supporting Information
Table S1: List of quantified proteins. Gene code and protein
name are shown in the columns ”Accession” and ”Description”,
respectively. The number of identified peptides, which may be shared
with other proteins, is shown in the column ”Peptide count”. Proteins
were quantified only when 3 or more non-redundant constituent peptides
were quantified, the number of which is shown in the column ”peptides
used for quantitation”. After normalisation, the average relative
abundance of each protein is shown. Individual comparisons between each
condition are also shown, with P being less tan 0.05. Raw data available
at https://github.com/HAHerrmann/FluxSampling
Col0Fum2/blob/master/ExperimentalData/ProteinConc.xlsx and stored under
the Zenodo DOI: 10.5281/zenodo.3366934
Figure S1: Concentrations of sucrose (a) and glucose (b) in
leaves, measured at the beginning (open symbols) and end (closed
symbols) of the photoperiod in Col-0 (circles) and fum2.2(triangles) plants. Error bars show standard mean error.
Figure S2: Summary of the enzyme concentrations of the sucrose
synthesis pathway of Col-0 (white bars) and fum2.2 (grey bars) plants in
control conditions (solid colours) and on Day 7 of 4°C treatment
(hatched bars), as shown in the legend on the bottom left. TP (triose
phosphate), FBP (fructose-1,6-bisphosphate), F6P (fructose-6-phosphate),
G6P (glucose-6-phosphate), Tre6P (trehaolse-6-phosphate), G1P
(glucose-1-phosphate), UDPG (uridine diphosphate glucose), S6P
(sucrose-6-phosphate). Error bars represent the standard mean error,
with different letters indicating significantly different values
Figure S3: Flux sampling results obtained from the wild type
Col-0 (black) and fum2 (red) models for the production of malate
(a,f), fumarate (b,g), starch (c,h), export (d,i) and respiration (e,j)
as predicted under control condition constraints (a-e) and seven days of
4°C conditions (f-j).