Supporting Information
Table S1: List of quantified proteins. Gene code and protein name are shown in the columns ”Accession” and ”Description”, respectively. The number of identified peptides, which may be shared with other proteins, is shown in the column ”Peptide count”. Proteins were quantified only when 3 or more non-redundant constituent peptides were quantified, the number of which is shown in the column ”peptides used for quantitation”. After normalisation, the average relative abundance of each protein is shown. Induvidual comaprsions between each condition are also shown, with P being less tan 0.05. Data also available at https://github.com/HAHerrmann/FluxSampling Col0Fum2/blob/master/ExperimentalData/ProteinConc.xlsx and stored under the Zenodo DOI: 10.5281/zenodo.3366934
Figure S1: Concentrations of sucrose (a) and glucose (b) in leaves, measured at the beginning (open symbols) and end (closed symbols) of the photoperiod in Col-0 (circles) and fum2.1 (triangles) plants. Error bars show standard mean error.
Figure S2: Summary of the enzyme concentrations of the sucrose synthesis pathway of Col-0 (white bars) and fum2.1 (grey bars) plants in control conditions (solid colours) and on Day 7 of 4°C treatment (hatched bars), as shown in the legend on the bottom left. TP (triose phosphate), FBP (fructose-1,6-bisphosphate), F6P (fructose-6-phosphate), G6P (glucose-6-phosphate), Tre6P (trehaolse-6-phosphate), G1P (glucose-1-phosphate), UDPG (uridine diphosphate glucose), S6P (sucrose-6-phosphate). Error bars represent the standard mean error, with different letters indicating significantly different values
Figure S3: Flux sampling results obtained from the Col-0 (black) and fum2.1 (gray) models for the production of malate (a,f), fumarate (b,g), starch (c,h), export (d,i) and respiration (e,j) as predicted under control condition constraints (a-e) and seven days of 4°C conditions (f-j).