2.10 Oral glucose tolerance test
Oral glucose tolerance test was performed according to the reported method of Kumar et al., The rats were divided into five groups of six rats in each group. Gp I served as control and received distilled water only; Gp II, Gp III, Gp IV, Gp V received the oral dose of PA 50, 100 and 200 mg/kg and glibenclamide (2.5 mg/kg), respectively. All the group rats received the 2 gm/kg glucose and after 60 min oral dose administration of pre-determined treatment. For the estimation of BGL, blood samples were collected via puncturing the retro-orbital plexus (0h) and every 30 min interval received the BGL of all group rats till 120 min (Ahmed et al., 2013, 2014c, 2015; Kumar et al., 2014).