3.7 Effect of PA extract on antioxidant parameters
Supplementary table 8 illustrates the effect of PA and glibenclamide on the antioxidant parameter in the STZ induced DM rats. The antioxidant parameters such as LPO, CAT, SOD, and GPx were scrutinized in all experimental animals. The level of antioxidant parameters was same in normal and normal received PA (200 mg/kg) group rats. STZ induced DM group revealed the increased level of LPO (15.5±1.84 µmole of MDA/mg protein) and reduce the level of SOD (14.54±3.01 units/mg protein), CAT (43.57±4.37 µmole of H2O2 consumed/min/mg of protein) and GPx (13.34±2.14 µmole of GSH/mg protein), respectively. STZ induced DM group treated with PA (200 mg/kg) showed the increase level of LPO (7.81±1.45 µmole of MDA/mg protein) and reduction in the level of SOD (43.65±2.05 units/mg protein), CAT (67.98±3.04 µmole of H2O2 consumed/min/mg of protein) and GPx (35±1.76 µmole of GSH/mg protein). STZ induced DM group treated with the glibenclamide demonstrate the increased level of LPO (7.98±1.23 µmole of MDA/mg protein) and reduction in the level of SOD (42.98±2.65 units/mg protein), CAT (66.71±2.93 µmole of H2O2consumed/min/mg of protein) and GPx (34.06±1.34 µmole of GSH/mg protein).
In STZ induced type II DM, destroy the pancreatic β-cells are destroyed as a result of, increase reactive oxygen species (ROS) and continuous generation of ROS that initiate the damage of tissues and react with the all cell membrane component and biological system, that lead to increased lipid peroxidation (LPO) reaction. The enhanced level of LPO modulate the membrane-bound enzymes activity and reduce the membrane fluidity, which results in damage to membrane fluidity (Ahmed et al., 2013, 2014c, 2015; Kumar et al., 2013, 2014). The increased level of LPO and decrease level of SOD, CAT, GPx were observed in the STZ induced DM group rats. Several researchers suggest that regular intake of antioxidant-rich food, increase antioxidant content reduces the LPO and enhanced the SOD, CAT, and GPx. SOD and CAT (Kumar et al., 2013, 2014; Ahmed et al., 2014c, 2015). SOD looks for and removes superoxide component from liver and converts to hydrogen peroxide CAT scavenges Hydrogen peroxide and detoxify the cells from free radical generators.