2.5 Insilico study
Insilico docking studies were performed for understanding binding
character and mode of rutin at the molecular level. Maestro (Schrodinger
software suite, version 9.4) was used for docking stimulation of ruin.
The ligprep application was further employed d for preparation of ligand
3D format via using build panel. Protein was obtained from protein data
bank (PDB ID: 5EQG, 2G63) this study n was prepared via eliminating the
hydrogen, solvent, and minimization of prepared legend. Validation was
made by the standard drug (glibenclamide) to re-docked at the protein
catalytic site.