Analysis of response surfaces
The response surface was performed to scrutinize the various parameters
for extraction of phyto-constituent from the plant extract. The
mathematical model generated the experimental data with the quadratic
polynomial equation for each response. Mathematical equation 1 and 2
summaries the relationship between the studied factors on the response
variables.
DPPH=
69.60+9.38×A+8.75×B+7.88×C+2.75×AB+0.5000×AC+1.25×BC+0.7000×A2-4.55×B2-3.30×C2——————————————————————————————1
ABTS=
61.60+10.13×A+8.38×B+7.50×C+3.00×AB+0.7500×AC+0.7500×BC-0.8000×A2-6.30×B2-3.55×C2———————————————————————–2
The surface response plots are the best way to represents the effect of
any independent on the free radicals via generating the surface response
plot of the model, which were generated via differentiation of two
variable within the experimental range under investigation and holding
the other variable at its central level (0 level). Supplementary figure
1 exhibited the three-dimensional plot, which represents the effect of
concentration solvent and temp on the scavenging the DPPH free radicals
on the extract of PA. On the resultant, we found that the solvent
concentration having the higher effect on the extraction of the PA
extract as a comparison with time effect. The supplementary figure 1A
and 1B represent that the increase the concentration of solvent increase
the scavenging activity of DPPH free radical. Because enhance the
solvent concentration encouraged the cell membrane break-down that
boosting the permeability of solvent into the solid matrix. On the other
hand, a high concentration of methanol, the start was appropriate for
the extraction of free radicals scavengers from the PA extract. In the
supplementary figure, 1C and 1D exhibited that increase the time for
extraction of plant extract increase the antioxidant activity via
scavenging the DPPH free radical. Supplementary figure 1E and 1F
demonstrated the increase the solvent concentration up-regulate the
extraction of phyto-constituents from the plant extract and the same
result was observed in the up-regulation in the time for the extraction
process. According to the supplementary figure 1E and 1F, it was found
that both temp and time influenced concurrently free radical scavenging
capacity. The extraction time plays a considerable role, which
influenced the PA extraction and hence free radical scavenging effect.
The mass transfer increase with increasing the time until the maximum of
extraction was achieved. Several research exhibited that the increase
the time extraction, its start the decomposition of active
phyto-constituents. A similar trend was observed in supplementary figure
2, which exhibited the effect of solvent concentration, temperature and
time on the scavenging activity of PA extract. On the resultant, we
observed that the solvent concentration and temp play a crucial role in
the extraction of plant extract.