2.12 Biochemical analysis
The BGL was determined by glucoseoxidase–peroxidase (GOD-POD) method by
blood glucose level kits. The plasma insulin level was estimated by
standard kits. The biological parameters such as glucose-6-phosphatase,
hexokinase and fructose-16-bisphosphatase were determined as per the
reported method of Kumar et al., Ahmed et al., Hepatic parameters such
as Serum glutamic oxalo acetic transaminase (SGOT), Serum glutamic
pyruvic transaminase (SGPT) and Alkaline Phosphatase (ALP) were
estimated by diagnostic kits (Span diagnostic). Lipid profiles such as
high-density lipoprotein (HDL), triglyceride (TG), total cholesterol
(TC), were estimated by the diagnostic kits (Span diagnostic kits) and
very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) were
estimated via using the following formula.
The coronary risk and atherogenic index were estimated via using the
following formula
\begin{equation}
\text{At}h\text{erogenic}\ \text{index}=\frac{\text{Low}\ \text{density}\ \text{lipoprotein}\ ch\text{olesterol}}{\ h\text{ig}h-\text{density}\ \text{lipoprotein}\ ch\text{olesterol}\ }\nonumber \\
\end{equation}\begin{equation}
\text{Coronary}\ \text{risk}\ \text{index}=\frac{\text{Total}\ ch\text{olesterol}}{\ h\text{ig}h-\text{density}\ \text{lipoprotein}\ ch\text{olesterol}\ }\nonumber \\
\end{equation}HOMA IR and HOMA-β were estimated via using the previously reported
protocol of Ahmed et al., with slight modification (Ahmed et al., 2014a,
2015).