2.6 α-glucosidase activity assay
The α-glucosidase inhibitory effect of PA extract was determined as described by Toma et al., with minor modification (Toma et al., 2014). For the estimation of α-glucosidase inhibitory effect, different concentration (10-200 μg/ml) of PA extract was prepared and 5 Mm solution of the substrate were incubated in the 96 well microplate and the mixture was subjected to at 37°C for 10 min. After 10 min, 0.8 U/ml α-glucosidase (prepared in 0.01 MPBS) was added and again incubated for 15 min. After 15 min, the reaction was terminated by addition of Na2CO3 (0.2M) prepared in PBS with the determination of absorbance at 405 nm. All the data presented as % inhibition from the following formula:
\begin{equation} \%\ \text{in}h\text{ibition}=\frac{(\text{Absorbance}\ \text{of}\ \text{Control}-\ \text{Absorbance}\ \text{of}\ \text{Test})}{\text{Absorbance}\ \text{of}\ \text{Control}}\times 100\nonumber \\ \end{equation}