3.7 Effect of PA extract on antioxidant parameters
Supplementary table 8 illustrates the effect of PA and
glibenclamide on the antioxidant parameter in the STZ induced DM rats.
The antioxidant parameters such as LPO, CAT, SOD, and GPx were
scrutinized in all experimental animals. The level of antioxidant
parameters was same in normal and normal received PA (200 mg/kg)
group rats. STZ induced DM group revealed the increased level of LPO
(15.5±1.84 µmole of MDA/mg protein) and reduce the level of SOD
(14.54±3.01 units/mg protein), CAT (43.57±4.37 µmole of
H2O2 consumed/min/mg of protein) and GPx
(13.34±2.14 µmole of GSH/mg protein), respectively. STZ induced DM group
treated with PA (200 mg/kg) showed the increase level of LPO
(7.81±1.45 µmole of MDA/mg protein) and reduction in the level of SOD
(43.65±2.05 units/mg protein), CAT (67.98±3.04 µmole of
H2O2 consumed/min/mg of protein) and GPx
(35±1.76 µmole of GSH/mg protein). STZ induced DM group treated with the
glibenclamide demonstrate the increased level of LPO (7.98±1.23 µmole of
MDA/mg protein) and reduction in the level of SOD (42.98±2.65 units/mg
protein), CAT (66.71±2.93 µmole of H2O2consumed/min/mg of protein) and GPx (34.06±1.34 µmole of GSH/mg
protein).
In STZ induced type II DM, destroy the pancreatic β-cells are destroyed
as a result of, increase reactive oxygen species (ROS) and continuous
generation of ROS that initiate the damage of tissues and react with the
all cell membrane component and biological system, that lead to
increased lipid peroxidation (LPO) reaction. The enhanced level of LPO
modulate the membrane-bound enzymes activity and reduce the membrane
fluidity, which results in damage to membrane fluidity (Ahmed et al.,
2013, 2014c, 2015; Kumar et al., 2013, 2014). The increased level of LPO
and decrease level of SOD, CAT, GPx were observed in the STZ induced DM
group rats. Several researchers suggest that regular intake of
antioxidant-rich food, increase antioxidant content reduces the LPO and
enhanced the SOD, CAT, and GPx. SOD and CAT (Kumar et al., 2013, 2014;
Ahmed et al., 2014c, 2015). SOD looks for and removes superoxide
component from liver and converts to hydrogen peroxide CAT scavenges
Hydrogen peroxide and detoxify the cells from free radical generators.