Analysis of response surfaces
The response surface was performed to scrutinize the various parameters for extraction of phyto-constituent from the plant extract. The mathematical model generated the experimental data with the quadratic polynomial equation for each response. Mathematical equation 1 and 2 summaries the relationship between the studied factors on the response variables.
DPPH= 69.60+9.38×A+8.75×B+7.88×C+2.75×AB+0.5000×AC+1.25×BC+0.7000×A2-4.55×B2-3.30×C2——————————————————————————————1
ABTS= 61.60+10.13×A+8.38×B+7.50×C+3.00×AB+0.7500×AC+0.7500×BC-0.8000×A2-6.30×B2-3.55×C2———————————————————————–2
The surface response plots are the best way to represents the effect of any independent on the free radicals via generating the surface response plot of the model, which were generated via differentiation of two variable within the experimental range under investigation and holding the other variable at its central level (0 level). Supplementary figure 1 exhibited the three-dimensional plot, which represents the effect of concentration solvent and temp on the scavenging the DPPH free radicals on the extract of PA. On the resultant, we found that the solvent concentration having the higher effect on the extraction of the PA extract as a comparison with time effect. The supplementary figure 1A and 1B represent that the increase the concentration of solvent increase the scavenging activity of DPPH free radical. Because enhance the solvent concentration encouraged the cell membrane break-down that boosting the permeability of solvent into the solid matrix. On the other hand, a high concentration of methanol, the start was appropriate for the extraction of free radicals scavengers from the PA extract. In the supplementary figure, 1C and 1D exhibited that increase the time for extraction of plant extract increase the antioxidant activity via scavenging the DPPH free radical. Supplementary figure 1E and 1F demonstrated the increase the solvent concentration up-regulate the extraction of phyto-constituents from the plant extract and the same result was observed in the up-regulation in the time for the extraction process. According to the supplementary figure 1E and 1F, it was found that both temp and time influenced concurrently free radical scavenging capacity. The extraction time plays a considerable role, which influenced the PA extraction and hence free radical scavenging effect. The mass transfer increase with increasing the time until the maximum of extraction was achieved. Several research exhibited that the increase the time extraction, its start the decomposition of active phyto-constituents. A similar trend was observed in supplementary figure 2, which exhibited the effect of solvent concentration, temperature and time on the scavenging activity of PA extract. On the resultant, we observed that the solvent concentration and temp play a crucial role in the extraction of plant extract.