DNA template preparation
Polyplax serrata lice (n=25) were collected from yellow-necked
wood mice (Apodemus flavicollis) captured in Czech Republic
(Struzna) and Germany (Baiersbronn) during 2011. Hoplopleura
acanthopus lice (n=40) were obtained from common voles (Microtus
arvalis) trapped in Czech Republic (Hlinsko) during 2014. All samples
were stored in 96% ethanol at -20°C. Total DNA was extracted from whole
louse abdomens using QIAamp DNA Micro Kit (Qiagen, Hilden, Germany)
according to manufacturer protocol. DNA quality was assessed by gel
electrophoresis and its’ concentration measured with Qubit High
sensitivity kit.