Contributions of plasticity and evolution to total metabolomic changes in time
For the positive ion mode, ancestral plasticity had about an equal contribution to the total metabolomic change compared to the evolutionary components during both transitions in fish predation (47.3% in the first transition and 55.9% in the second transition, Figure 5a-b). Of the two evolutionary components, the contribution of evolution of plasticity was larger (31.0% and 24.6%) compared to constitutive evolution (21.7% and 19.4%) during both transitions. The results were highly similar for the negative ion mode: ancestral plasticity had about an equal contribution compared with evolution during both transitions (46.5% and 48.8%), and the evolution of plasticity contributed more (30.4% and 30.0%) than constitutive evolution (23.1% and 21.2%, Figure 5c-d).