3.2.3 Effect of Lipase Concentration
Table 3.3 shows the FFA content of PFAD and concentration of vitamin E,
as a function of lipase concentration in the reaction mixture. The
system parameters were set at a temperature of 65 \(\pm\) 1 , with
70% v/w water content and reaction time of 5hr. FFA content liberated
and vitamin E extracted increased steadily as the amount of lipase used
for hydrolysis increased. In series of hydrolysis performed, the peak
values of FFA content liberated and vitamin E extracted were 93.48% and
7.04% respectively at lipase concentration of 10.5%, and beyond this,
hydrolysis extent did not increase much even though more lipase was
used. This could be caused by unavailability of substrate, or the number
of units of substrates per unit of lipase was relatively low when the
concentration of lipase increased. Similar changes were observed in
vitamin E concentration extracted from the samples. Vitamin E initially
increased significantly with increase of FFA levels in the PFAD when
lipase concentration in the reactant increased from 0.5 to 10.5% w/w.
Further increase in lipase concentration to 13.5% did not significantly
increase the vitamin E concentration extracted from the samples.
Table 3.3 Changes in percentages of free fatty acid (FFA) and vitamin E
extracted as a function of lipase concentration in the reaction mixture.