3.3 Optimization of Enzymatic Hydrolysis Process
Response surface methodology (RSM) was the technique applied to optimize
the production of tocopherol concentrates from PFAD from the enzymatic
hydrolysis process. Several statistical tools within the RSM were
systematically utilized to evaluate the effects of process variables and
the interactions among them. Out of many designs available in the RSM,
the central composite design (CCD) was particularly used to optimize the
enzymatic hydrolysis process, and responses were predicted using the
optimization functions of the Design Expert (version 10) software.
For detection of tocopherol, the optimization of enzymatic hydrolysis
process set-up is shown in Table 2.1 and the experimental array is
displayed in the next section of this work (in terms of coded values).