2.2.5 Determination of Acylglycerols and Slip Melting Point
Quantitative separation of mono-, di, and tri- acylglycerols in the PFAD
and HNPFAD samples were determined following standard experimental
principles of the American Oil Chemist Society (ACOS, 1990) Recommended
Practice Cd 11c-93. The AOCS official methods Cc 3-25 was the
experimental method applied to determine the slip melting point of both
samples. Total fatty acid component present in the sample was the sum of
FFA and acylglycerols.