3.3.4 Optimization Result
About a hundred different optimized numerical solutions were suggested by the Design Expert Software. Thus, H2O weight of 60.604 %(v/w), lipase weight of 7.130 %(w/w) and reaction time of 2.713 hrs gave the best (at optimized state) experimental protocol for the enzymatic hydrolysis PFAD in terms of yield of total tocopherol (as vitamin E) with a desirability of unity.