2.2.5 Determination of Acylglycerols and Slip Melting Point
Quantitative separation of mono-, di, and tri- acylglycerols in the PFAD and HNPFAD samples were determined following standard experimental principles of the American Oil Chemist Society (ACOS, 1990) Recommended Practice Cd 11c-93. The AOCS official methods Cc 3-25 was the experimental method applied to determine the slip melting point of both samples. Total fatty acid component present in the sample was the sum of FFA and acylglycerols.