2.7 Data Analysis
Triplicate samples were analysed and the mean values calculated for each physicochemical parameter determined. Mean and standard deviations physicochemical parameters of samples were calculated using MS excel. Identification of FFAs and vitamin E in PFAD and HNFAD samples were performed by retention time and comparison of product component of known FAME standard (palmitic, oleic, stearic, and linoleic acids) with GC-MS analysis.