3.3 Optimization of Enzymatic Hydrolysis Process
Response surface methodology (RSM) was the technique applied to optimize the production of tocopherol concentrates from PFAD from the enzymatic hydrolysis process. Several statistical tools within the RSM were systematically utilized to evaluate the effects of process variables and the interactions among them. Out of many designs available in the RSM, the central composite design (CCD) was particularly used to optimize the enzymatic hydrolysis process, and responses were predicted using the optimization functions of the Design Expert (version 10) software.
For detection of tocopherol, the optimization of enzymatic hydrolysis process set-up is shown in Table 2.1 and the experimental array is displayed in the next section of this work (in terms of coded values).