2.1 Reagents and Sample Collection
All chemicals, reagents, tocopherol and fatty acid methyl ester (FAME) standards, and solvents used were from the Multi-Purpose laboratory, Ahmadu Bello University, Zaria. Fatty acid distillate from palm oil (PFAD) was purchased from Presco Palm Oil Processing Industry located at Obaretin, Edo State in Nigeria. The PFAD sample was stored in the refrigerator at a temperature of \(\leq\)4 till further analyses.