2.6 Tocopherols Quantification
An external calibration was performed prior to analyses of edible plant oil by injecting different concentrations on the column. Standard curves (peak area vs concentration) were calculated by linear regression analysis. Injections in triplicate were made at each concentration for both standards and samples. The calibration curves were constructed using standard solutions of \(\alpha,\ \beta,\ \gamma\ \)and \(\delta\)- tocopherols and used for quantification. The total tocopherol content is expressed as milligram per gram FAD (mg/g FAD).