Bioinformatic filter Number of reads Number of sequences Number of taxa assignations
Sequences with < 10 reads discarded 299,479,007 14,423 765
Assignation to fish taxa only 226,630,600 10,521 719
Sequence length between 50 & 75 bp 226,391,708 10,408 719
PCR errors removed 214,864,059 9,345 714
Taxa assignation (order>80%) 181,034,672 7,389 678