Bioinformatic filter Number of reads Number of OTUs
No filter 331,839,591 183,546
OTUs with < 10 reads discarded 331,427,418 4,012
Assignments to non-fish taxa removed 252,276,717 2,737
OTUs length between 50 & 75 bp 251,635,415 2,643
PCR errors removed 228,944,832 1,252
LULU algorithm curation 228,569,519 972
Assignment to families (similarity > 85%) 174,357,646 819