Fluorescence assay
FITC- or HiLyte-Fluor-488-labeled proteins in free or
platelet-bound form were assayed by measuring fluorescence of their
solutions or suspensions in 300 µl PBS in Nunc Polysorb BREAKAPART
microplates using BIOTEK FLx800 fluorescence reader employing excitation
at 485 nm and emission at 528 nm.