2.2.7 Interpretation of the results
For the single-color fluorescence method, if the ΔCT value was greater
than 5, the sample was considered to be homozygous (the mutation
amplification curve is in front, which is homozygous; the wild-type
amplification curve is in front, which is wild-type), and if the ΔCT
value was less than 5, the sample was considered to be heterozygous. For
the two-color fluorescence method, according to the probe-labeled
fluorescence signal, the mutation sites could be identified, but
homozygous and heterozygous mutations could not be distinguished.