2.2.7 Interpretation of the results
For the single-color fluorescence method, if the ΔCT value was greater than 5, the sample was considered to be homozygous (the mutation amplification curve is in front, which is homozygous; the wild-type amplification curve is in front, which is wild-type), and if the ΔCT value was less than 5, the sample was considered to be heterozygous. For the two-color fluorescence method, according to the probe-labeled fluorescence signal, the mutation sites could be identified, but homozygous and heterozygous mutations could not be distinguished.