NCTD induced cells apotosis through Bcl-2 family and caspase family
In addition we investigated the expression levels of Bcl-2 and caspase families in NCTD-induced apoptosis. As show in Figure 4A, results revealed that Bcl-2 and Mcl-1 were markedly decreased after exposed to different concentrations of NCTD for 24 h. whereas the expressions levels of Bax and Bcl-xl, did not change significantly. Furthermore As shown in Figure 4B, the protein expressions levels caspase-3, caspase-9 and PARP decreased, however pro-caspase-8 has not significantly changed. in Collectively, NCTD induced A549 cell apoptosis via the mitochondrial signaling pathway.
fig.4