Figure Legends
Figure 1 Map of South Africa showing locations of successful (circles; n
= 8) and unsuccessful (triangles; n = 2) sample collection from 10
mountain ranges across the distribution of Chaetops frenatus(shaded grey area outlined in dark red), and from two sites (squares; n
= 2) within the distribution of the C. aurantius (shaded grey
area outlined in orange). Numbers in parentheses indicate sample number
collected from each range. Samples were collected from Oct 2016 to Oct
2017 in the Western and Eastern Cape provinces of South Africa.
Figure 2 Minimum-spanning haplotype networks for mitochondrial loci cytb
and ND2. Each circle represents a haplotype with its size proportional
to haplotype frequency, each branch illustrates one nucleotide change
and each hash-mark indicates an additional nucleotide difference.
Circles are colour-coded according to sampling locality. Distributions
of Chaetops frenatus and C. aurantius are depicted by
outlined shaded areas in red and orange respectively.
Figure 3 Consensus tree based on maximum likelihood inference for three
genetic markers (two mtDNA: cytb and ND2; one nDNA: RAG1), fromChaetops frenatus (n = 71), with outgroups C. aurantius (n
= 15) and Picathartes gymnocephalus (n = 2). Bootstrap scores of
>50 are indicated on each branch. Inset: ND2 haplotype
frequencies showing relative frequency per locality of each haplotype
(C. frenatus haplotypes n = 15, ”A” through ”N”; C.
frenatus haplotypes n = 5, ”O” through ”S”). Localities are indicated
on inset map of South Africa with species distributions shaded in grey
and outlined in red (C. frenatus ) or orange (C.
aurantius ).
Figure A1 Consensus tree based on Bayesian inference for three genetic
markers (two mtDNA: cytb and ND2; one nDNA: RAG1), from Chaetops
frenatus (n = 71), with outgroups C. aurantius (n = 15) andPicathartes gymnocephalus (n = 2). Bootstrap scores of
>50 are indicated on each branch.