RT-qPCR
One μg of total RNA was reverse-transcribed into first-strand complementary DNA (cDNA) with Prime Script RT Reagent Kit (Perfect Real Time, TaKaRa, Dalian, China) according to the manufacturer’s instructions. Real-time PCR was performed with 50ng of cDNA using One-Step SYBR PrimeScriptTM RT-PCR kit (Takara, Dalian, China). All reactions were performed in triplicate. All primers were initially evaluated for efficiency using relative standard curve and electrophoresis on gel. Primer sequences are listed in Supplementary Table 3.