Table 4 : Average Amount of AIEC colonies that survived under sequential therapy (Set A)
The AIEC bacteria that were treated via sequential therapy in set b were not able to replicate nearly as well as the bacteria that were treated via combination therapy. This is because during sequential therapy, specifically in set b, select antibiotics in sequential order were able to the destroy DNA of AIEC bacteria and prevent them from multiplying (10). Set b was also found to be more effective than set a because since the strong antibiotics were applied first, this eliminated most bacterial colonies before they have a chance to evolve. This was a much more effective method than the weak-strong treatment and yielded a much lower number of surviving colonies.
LB+ Kan then Spe (Plate #1) LB+ AMP then Spe (Plate #2) LB+ Kan/Cam then Spe (Plate #3) LB+P/S then Spe (Plate #4) LB+ P/S then Kan (Plate #5) LB+P/S then AMP (Plate #6) LB Agar (Control) (Plate #7)
AIEC Colonies (Original Set B Plates) 19 14 0 0 0 0 34
AIEC Colonies (Replicate #l of Set B Plates) 17 13 0 0 0 0 31
AIEC Colonies (Replicate #2 of Set B Plates) 19 12 0 0 0 0 33