In order to determine whether the LR neurons are a truly distinct cell type, we sought to identify the physiological properties that can clearly distinguish them from other neurons in the superficial RSG. Specifically, using features such as rheobase, input capacitance, input resistance, and spike width, we were able to isolate the LR cells from both FS and RS cells (Figure 2). LR cells cluster clearly and separately from FS and RS cells based on these intrinsic properties, suggesting they are a unique and distinct subtype of neuron compared to the others included in this study.