Significance
Past work of the field has demonstrated that transcription at centromeres of various eukaryotes is an important and conserved event for centromere function and chromosomal integrity. As this topic of research progresses, it will be important to characterize the molecular mechanism of cenRNAs, which are not understood completely. This research lays groundwork for investigating further questions on the molecular mechanisms of cenRNA-mediated CENP-A loading to centromeres. In particular, the finding that centromere-localizing cenRNAs are of heterogeneous length and sequence identity invites one to think whether they could be involved in important RNA-RNA interactions (eg. RNAi-based methods) that are important for CENP-A loading.