PEGylated Paclitaxel Nanomedicine Meets 3D Confinement: Cytotoxicity and
Cell Behaviors
Abstract
It is beneficial to investigate the effect of nanomedicines on cancer
cell behaviors on three-dimensional (3D) platforms for evaluating and
developing novel antitumor nanomedicines in vitro.
Two-dimensional flat surface is widely used to study the cytotoxicity of
nanomedicines on cancer cells, and there is little work using 3D
confinement to assess the effect of nanomedicine on cancer cells. In
this work, PEGylated paclitaxel nanoparticles (PEG-PTX NPs) were applied
for the first time to treat nasopharyngeal carcinoma (NPC43) cells in 3D
confinement which consisted of microwells with different sizes and a
glass cover. The cytotoxicity of the small molecule drug paclitaxel
(PTX) and PEG-PTX NPs were studied in microwells with sizes of 50×50,
100×100, and 150×150 μm2 and with covers. After NPC43
cells were treated with PTX and PEG-PTX NPs, the cell migration speed
and cell morphology were analyzed in different microwells without and
with covers. The relationship between the cytotoxicity from drugs, the
size effect from microwells, and cell behaviors were analyzed. These
results not only show the effect of 3D confinement on cytotoxicity of
nanomedicines and cell behaviors, but also provide a novel method to
screen anticancer drugs and evaluate the cell behaviors in vitro.