Lipidomic analysis of moss species Bryum pseudotriquetrum and
Physcomitrium patens under cold stress
Abstract
As non-vascular plants, which lack lignin for protection, bryophytes
support themselves in harsh environment by producing various chemicals.
In response to cold stress, lipids play a crucial role in cell
adaptation and energy storage. Specifically, bryophytes survive at low
temperatures by producing very long-chain polyunsaturated fatty acids
(vl-PUFAs). However, a systematic knowledge and comprehensive
understanding of the cold acclimation of bryophytes is limited. To
overcome this obstacle, we performed lipid profiling using ultra-high
performance liquid chromatography-quadrupole time of flight mass
spectrometry (UHPLC-QTOF-MS) of two moss species ( Bryum
pseudotriquetrum and Physcomitrium patens) cultivated at
standard condition compared to those cultivated at cold stressed
condition. The potential biomarkers were identified by multivariate
statistical analysis in each species. In B. pseudotriquetrum, we
found that the phospholipids and glycolipids increased significantly
under cold stress, while storage lipids decreased. The accumulation of
the lipids with high unsaturation degrees (i.e. at least one fatty acyl
chain contains more than two double bonds) mostly appear in
phospholipids and glycolipids. However, this trend cannot be observed in
P. patens. This suggests that different moss species may undergo
a different lipid metabolic pathway of cold adaptation. Our findings
present a deeper understanding of how mosses are adapted to cold
temperature and provide a basis for future studies.