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Improving the efficacy of extended storage for reducing the risk of viral contaminated plant-based feed ingredients through investigating ambient temperature requirements.
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  • Nicholas Dee,
  • Karyn A. Havas,
  • Apoorva Shah,
  • Aaron Singrey,
  • Gordon Spronk,
  • Megan Niederwerder,
  • Eric Nelson,
  • Scott Dee
Nicholas Dee
University of Minnesota School of Public Health

Corresponding Author:[email protected]

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Karyn A. Havas
Pipestone County
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Apoorva Shah
Eden Prairie Schools
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Aaron Singrey
South Dakota State University Department of Veterinary and Biomedical Sciences
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Gordon Spronk
Pipestone County
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Megan Niederwerder
Kansas State University College of Veterinary Medicine
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Eric Nelson
South Dakota State University Department of Veterinary and Biomedical Sciences
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Scott Dee
Pipestone County
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Viruses of veterinary significance such as African swine fever virus, are known to survive for extended periods in plant-based feed ingredients imported into North America. To reduce the likelihood of virus introduction, high-risk ingredients, such as oil seed meals, are stored in designated facilities for extended periods under controlled environmental conditions to minimize viral infectivity prior to use in diets. While 30 days has become a standard storage period, the required ambient temperature to inactivate viruses during this time is not known. To address the question, 1-metric ton totes of conventional soybean meal were inoculated with PRRSV 144 lineage 1C variant and SVA prior to storage for 30 days at 23.9º C, 15.5º C, or 10º C, and feeding to pigs. Virus infectivity was evaluated through detection of viral RNA in oral fluid samples, along with clinical signs. Results indicated that inactivation of both viruses occurred in soy stored at 23.9º C. In contrast, SVA infectivity was observed in soy stored at both 15.5º C and 10º C, while PRRSV 144 L1C variant infectivity was only observed in soy stored at 10º C. These results suggest that a storage period of 30-days and a temperature of 23.9º C are required to reduce the risk of virus contaminated plant-based feed ingredients, such as soybean meal.
04 Feb 2022Submitted to Transboundary and Emerging Diseases
04 Feb 2022Submission Checks Completed
04 Feb 2022Assigned to Editor
08 Feb 2022Reviewer(s) Assigned
10 Mar 2022Review(s) Completed, Editorial Evaluation Pending
19 Mar 2022Editorial Decision: Revise Major
20 Mar 20221st Revision Received
20 Mar 2022Submission Checks Completed
20 Mar 2022Assigned to Editor
30 Mar 2022Review(s) Completed, Editorial Evaluation Pending
30 Mar 2022Editorial Decision: Accept