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Ultrasound assisted in-situ separation of sophorolipids in multi-phase fermentation system to achieve efficient production of Candida bombicola
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  • Yang Chen,
  • Xu Tang,
  • Ya Li,
  • Chang Liu,
  • Yingping Zhuang,
  • Xiwei Tian,
  • Ju Chu
Yang Chen
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering

Corresponding Author:[email protected]

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Xu Tang
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Ya Li
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Chang Liu
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Yingping Zhuang
East China University of Science & Technology
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Xiwei Tian
State KeyLaboratory of Bioreactor Engineering,
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Ju Chu
East China University of Science and Technology State Key Laboratory of Bioreactor Engineering
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Abstract

Sophorolipids (SLs) are regarded as one of the most promising biosurfactants. However, high production costs are the main obstacle to extended SLs application. Semi-continuous fermentation, which is based on in-situ separation, is a promising technology for achieving high SLs productivity. In this study, the sedimentation mechanism of SLs was analyzed. The formation of a hydrophobic mixture of SLs and rapeseed oil was a key factor in sedimentation. And the hydrophobicity and density of the mixture determined SLs sedimentation rate. On this basis, ultrasonic enhanced sedimentation technology (UEST) was introduced, by which the sedimentation rates were increased by 46.9% to 485.4% with different ratio of rapeseed oil to SLs. UEST-assisted real-time in-situ separation and semi-continuous fermentation were performed. SLs productivity and yield were 2.15 g/L/h and 0.58 g/g, respectively, simultaneously the loss ratio of cells, glucose, and rapeseed oil were significantly reduced. This study provides the new horizon for optimization of the SLs fermentation process.
01 Sep 2021Submitted to Biotechnology Journal
02 Sep 2021Submission Checks Completed
02 Sep 2021Assigned to Editor
15 Sep 2021Reviewer(s) Assigned
11 Oct 2021Editorial Decision: Revise Minor
20 Oct 20211st Revision Received
21 Oct 2021Submission Checks Completed
21 Oct 2021Assigned to Editor
21 Oct 2021Reviewer(s) Assigned
09 Nov 2021Editorial Decision: Revise Minor
14 Nov 20212nd Revision Received
15 Nov 2021Submission Checks Completed
15 Nov 2021Assigned to Editor
15 Nov 2021Editorial Decision: Accept
Feb 2022Published in Biotechnology Journal volume 17 issue 2 on pages 2100478. 10.1002/biot.202100478