This study was presented orally at the 6th International Congress on Advances in Bioscience and Biotechnology (ICABB), and published in the congress proceedings book as a full text with the acceptance of the reviewers.
Erdogan, H., Ozmen P., Bulbul, H.N. (2022, July). Antibacterial Efficacy of Alternative and
Conventional Endodontic Irrigants on S.mutans and E.faecalis. 6th International Congress
on Advances in Bioscience and Biotechnology (ICABB), Aksaray, Türkiye. Page:44-53
Antibacterial Efficacy of
Alternative and Conventional Endodontic Irrigants on S.mutans andE.faecalis
Objectives: Effective removal of microorganisms from the root
canal system plays an important role in the success of endodontic
treatment. Sodium hypochlorite and
chlorhexidine are commonly used
irrigation solutions in endodontics, but due to their disadvantages, the
search for alternative irrigation solutions continues. This study aimed
to evaluate the in vitro antibacterial activities of alternative and
conventional endodontic irrigation solutions against Enterococcus
faecalis and Streptococcus mutans.
Methods: The following solutions were evaluated: 5.25% sodium
hypochlorite (NaOCl); 2% chlorhexidine (CHX); 0.02% (200 ppm)
hypochlorous acid (HOCl); 0.1% Polyhexanide (PHMB). Sterile saline was
used as a negative control. Antiseptics were impregnated on disk-shaped
filter papers. Then, these discs were placed first separately and then
in pairs to investigate the synergistic effects of antiseptics when used
alone or together, and the zone diameters were measured by the Kirby
Bauer disc diffusion method.
Results: In S.mutans, 20mm inhibition zone diameter was
measured for both CHX and HOCl when used alone, and the highest
antimicrobial activity was observed. Approximately the same efficacy was
observed in NaOCl (6mm) and PHMB (5mm). The highest efficacy was
observed in CHX+HOCl (20mm) in combined use. In E.faecalis , the
highest antimicrobial activity was observed in CHX as a 5mm inhibition
zone diameter when used alone, and in CHX+HOCl (12mm) and CHX+PHMB
(10mm) when used in combination.
Conclusions: Alternative endodontic irrigation solutions had
different antimicrobial effects on test microorganisms. The use of
hypochlorous acid, which is an alternative irrigation solution, together
with chlorhexidine may provide microbiological advantages in clinical
use.
Keywords: Antimicrobials, chlorhexidine, endodontics,
irrigation, microbiology, sodium hypochlorite